Alejandra Bravo and Mario Soberon, a wife-husband research team at the National Autonomous University of Mexico (UNAM) who received sanctions — later lifted — for manipulating images in a number of papers have corrected another article.
The paper, “The mitogen-activated protein kinase p38 is involved in insect defense against Cry toxins from Bacillus thuringiensis,” appeared in Insect Biochemistry and Molecular Biology in 2010 and has been cited 23 times, according to Thomson Scientific’s Web of Knowledge. Here’s the correction notice:
The authors regret that Figure 1 and Figure 3 were edited without making clear that some of the images were merged images that came from different gels. The corrected Figure 1 and Figure 3 composed of original blots or replicas of the same experiments now makes clear that some of the images came from different gels. This correction does not affect the conclusions of the paper. The authors would like to apologise for any inconvenience caused.
Phosphorylation of MAPK p38 in insects after intoxication with Cry toxins. Panel A, first instar Manduca sexta larvae were intoxicated with 2 ng/cm2 (LC50) or 20 ng/cm2 Cry1Ab for up to 1 h. M. sexta larvae were also treated 1 h with the non-toxic mutant Cry1Ab-R99E affected in oligomerization and pore formation. Panel B, fourth instar Aedes aegypti larvae were fed an LC50 of Cry11Aa toxin or the non-active mutant Cry11Aa-R90E. The presence of phosphorylated and total MAPK p38 proteins was analyzed by western blot using specific antibodies. The blots presented here are representative figures of three independent experiments. Numbers under the blots are percentage in relation to the control band (no toxin or time 0, which was considered as 100%), after scanning the bands. Ph-p38, phosphorylated MAPK p38; p38, total MAPK p38.
Silencing of MAPK p38 by RNAi in Manduca sexta and Aedes aegypti larvae. MAPK p38 expression was silenced by feeding dsRNA to M. sexta and A. aegypti larvae. Panel A, The presence of total MAPK p38 protein was analyzed by western blot assays using specific antibodies. Panel B, The expression of MAPK p38 gene was analyzed by RT-PCR assays. Numbers under the bands are percentage in relation to the control band, after densitometry analysis. The control bands correspond to non-silenced larvae, which were labeled with a C and were considered as 100%.
The university’s findings referred to eleven papers. This is the third correction we’ve seen.