The Journal of Veterinary Medical Science has retracted an article in published as an early online paper back in August. The paper: “Incidence Study of Brucella abortus and Brucella melitensis in Bovine and Buffalo Semen Samples by Real-Time PCR Assay in Iran.”
This article released online on August 3, 2012 as advance publication was withdrawn from consideration for publication in The Journal of Veterinary Medical Science at author’s request.
Per the abstract:
Advances in molecular sciences allow us to make extensive use of equipment in order to meet needs. In this present study to overcome the shortcomings of culture and serological methods for detection and identification of Brucella abortus and Brucella melitensis in bovine and buffalo semen samples, a conventional and real-time PCR assays were used. All samples were collected and transferred to laboratory, genomic DNA was extracted and the molecular methods by specific primers were performed. In total 241 (23.86%) bovine and 261 (26.48%) buffalo semen samples were positive for presence of Brucella species by culture method. After single step PCR it was recognized that 255 (25.24%) bovine and 271 (28.28%) buffalo semen samples were positive for incidence of Brucella species by conventional PCR. After using of real-time PCR, it was recognized that 156 (15.44%) and 63 (6.23%) bovine and 188 (19.62%) and 58 (6.05%) of buffalo semen samples were infected by Brucella abortus and Brucella melitensis, respectively. Beside, the results of real-time PCR showed that 3.56 and 2.27% of bovine and 2.6 and 1.87% of buffalo semen samples were infected with unknown species and both species, respectively. The sensitivity and specificity of conventional PCR were obtained 100 and 98.17% in bovine and 100 and 98.56% in buffalo semen samples. The CT values had significant differences (P<0.05) between the incidences of both Brucella abortus and Brucella melitensis in bovine with buffalo semen samples.